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Provided that the plant has an adequate supply of water, the water which is lost through the stomata is replaced by water from the xylem vessels in the leaf. In fact, evaporation of water molecules from the cells of a leaf creates a suction which pulls water from the xylem cells of roots. The loss of water in the form of vapour from the aerial parts of the plant is known as transpiration.

Thus, transpiration helps in the absorption and upward movement of water and minerals dissolved in it from roots to the leaves. It also helps in temperature regulation. The effect of root pressure in transport of water is more important at night. During the day when the stomata are open, the transpiration pull becomes the major driving force in the movement of water in the xylem.